BLOOD

2) LEUKOCYTES (white blood cells)

Most leukocytes are located in peripheral tissues rather than in the blood, only about 2% are actually in the blood. They travel through blood looking for disturbances in your body's normal functioning. If they detect the appropriate signal, demonstrate both chemotaxis (the ability to move towards the signal) and emigration or diapedesis (they can fit between small spaces between cells and pass through capillary walls due to their ability to move in an amoeboid fashion). Microbe toxins, colony stimulating factors, & kinins (produced by damaged tissues) may attract leukocytes (especially neutrophils & macrophages).

Some leukocytes (neutrophils, macrophages, monocytes) phagocytize (engulf) microbes and may kill with chemicals such as lysozyme, defensins, & strong oxidants. Eosinophils undergo the exocytosis of these chemicals onto parasites that are too large for phagocytosis. Basophils facilitate the inflammatory response. Most leukocytes live only a few days and during an infection they may only survive a few hours. Because they are so short-lived, 75% of blood cells made by the bone marrow (perhaps 4-6 million per second are leukocytes.

Neutrophil

neutrophil neutrophil

Eosinophil

eosinophil eosinophil

Basophil

basophil

Monocyte (which mature into macrophages)

monocyte monocyte

Lymphocyte

lymphocyte lymphocyte

 


The types of white blood cells have fixed percentages in the blood and a differential white blood cell count examines these percentages. Changes from normal values may be useful in diagnosing a patient's problem:


--neutrophils increase due to burns, stress, inflammation, & bacterial infections; decrease after radiation and in B12 deficiencies
--eosinophils increase due to allergies, parasitic infections, and autoimmune diseases; decrease in stress and Cushings syndrome
--basophils: increase in allergies, hypersensitivities, cancers, hypothyroidism; decrease during pregnancy, ovulation, stress, hyperthyroidism
--lymphocytes: increase in viral infections, immune disorders, some leukemias
--monocytes: increase in viral or fungal infections, tuberculosis


Leukemia is the uncontrolled production and accumulation of immature leukocytes; since this decreases the numbers of red blood cells and platelets made, leukemia patients are in danger of anemia, hemorrhaging, and infection.

Note the abnormally high numbers of white blood cells (staining purple) in the blood from a leukemia patient in the following images.
leukemia leukemia

3) PLATELETS:

In the bone marrow, very active cells known as metamegakaryocytes shed bits of cytoplasm surrounded by cell membrane into the bloodstream, these are known as platelets. The average metamegakaryocyte produces 1000-1500 platelets and there are 250,000-400,000 platelets per ml of blood. They are only 2-4 um in size and have no nucleus. They are full of granules that have chemicals to promote clotting. They live only about 5-9 days and are visible as the small purple cells between the red blood cells in the following images..

plateletsplateletplatelets
Normally, platelets are repelled from each other and the blood vessel walls.

platelets

Platelets have 3 functions after bleeding begins: they form a platelet plug to block the break in the blood vessel, they induce coagulation (form a fibrin clot), and they help maintain endothelial lining of blood vessels. After vascular damage has occurred, platelets release their granules, form a platelet plug (in the first image below), and induce the blood protein fibrinogen to form the fibrin of the blood clot (second image).

clotting

clotting


Fibrinolysis is the dissolution of clots; no clot is meant to last forever. Clots which have formed inappropriately can be dissolved as well.


Anything floating in the blood (even an air bubble) that may potentially block a blood vessel is called an embolus. Inappropriate clots may form such plugs in undamaged blood vessels in a process known as thrombosis.
Thrombolytic agents (kallikrein, tPA, streptokinase) can be injected into the body to dissolve clots. Anticoagulants (such as heparin and warfarin) prevent blood from clotting in the first place
Platelets may start the formation of clots in response to irregular endothelial surfaces, artificial heart valves, or endothelial lipid plaques
Low daily doses of aspirin decreases the formation of a thrombus (by inhibiting the clotting factor thromboxane) and thus decrease the risk of heart attack and stroke.
Hemophilia is a genetic disease caused by the inability to make a clotting factor. Hemophiliacs are in danger of hemorrhage if they are cut. There are different kinds of hemophilia but each involves the lack of a clotting factor. Hemophilia types A and B are sex-linked while type C isn't. Tragically, most hemophiliacs who received blood transfusions through 1982-85 were contaminated with HIV.